Journal: Frontiers in Oncology

Article Title: Discovery of Pancreatic Ductal Adenocarcinoma-Related Aberrant Glycosylations: A Multilateral Approach of Lectin Microarray-Based Tissue Glycomic Profiling With Public Transcriptomic Datasets

doi: 10.3389/fonc.2020.00338

Figure Lengend Snippet: Differential glycomic profiling of PDAC tissue sections. (A) Representative images of non-tumor and tumor regions in hematoxylin and eosin-stained frozen sections. The arrows indicate the cancerous ducts. (B) Schematic overview of lectin microarray-based glycomic profiling of unstained frozen sections.

Article Snippet: The adjusted Cy3-labeled protein samples were applied to a lectin microarray slide (LecChip TM Ver.1.0; GlycoTechnica Ltd., Yokohama, Japan) prewashed with the probing buffer and incubated for overnight at 20°C with gently shaking to completely form the lectin–glycoprotein complex.

Techniques: Staining, Microarray

Journal: Frontiers in Oncology

Article Title: Discovery of Pancreatic Ductal Adenocarcinoma-Related Aberrant Glycosylations: A Multilateral Approach of Lectin Microarray-Based Tissue Glycomic Profiling With Public Transcriptomic Datasets

doi: 10.3389/fonc.2020.00338

Figure Lengend Snippet: Summary of univariate analysis for tissue glycomic profiles of tumor (T) and non-tumor (N) regions.

Article Snippet: The adjusted Cy3-labeled protein samples were applied to a lectin microarray slide (LecChip TM Ver.1.0; GlycoTechnica Ltd., Yokohama, Japan) prewashed with the probing buffer and incubated for overnight at 20°C with gently shaking to completely form the lectin–glycoprotein complex.

Techniques:

Journal: Frontiers in Oncology

Article Title: Discovery of Pancreatic Ductal Adenocarcinoma-Related Aberrant Glycosylations: A Multilateral Approach of Lectin Microarray-Based Tissue Glycomic Profiling With Public Transcriptomic Datasets

doi: 10.3389/fonc.2020.00338

Figure Lengend Snippet: Multivariate analysis of the tissue glycomic profiles by PCA. In the score plot ( left panel ), the lectin microarray data of the non-tumor (blue) and tumor (red) regions of PDAC tissue sections ( N = 14) were plotted. The numbers of three non-tumor samples obtained from O blood group patients (No. 11–13) are indicated. In the loading plot ( right panel ), the relative signal intensities of 45 lectins on the array are plotted. The lectins extracted for the non-tumor and tumor regions are indicated in blue and red, respectively.

Article Snippet: The adjusted Cy3-labeled protein samples were applied to a lectin microarray slide (LecChip TM Ver.1.0; GlycoTechnica Ltd., Yokohama, Japan) prewashed with the probing buffer and incubated for overnight at 20°C with gently shaking to completely form the lectin–glycoprotein complex.

Techniques: Microarray

Journal: Frontiers in Oncology

Article Title: Discovery of Pancreatic Ductal Adenocarcinoma-Related Aberrant Glycosylations: A Multilateral Approach of Lectin Microarray-Based Tissue Glycomic Profiling With Public Transcriptomic Datasets

doi: 10.3389/fonc.2020.00338

Figure Lengend Snippet: Summary of univariate analysis for glycomic profiles of basigin from tumor (T) and non-tumor (N) regions.

Article Snippet: The adjusted Cy3-labeled protein samples were applied to a lectin microarray slide (LecChip TM Ver.1.0; GlycoTechnica Ltd., Yokohama, Japan) prewashed with the probing buffer and incubated for overnight at 20°C with gently shaking to completely form the lectin–glycoprotein complex.

Techniques:

Journal: Frontiers in Oncology

Article Title: Discovery of Pancreatic Ductal Adenocarcinoma-Related Aberrant Glycosylations: A Multilateral Approach of Lectin Microarray-Based Tissue Glycomic Profiling With Public Transcriptomic Datasets

doi: 10.3389/fonc.2020.00338

Figure Lengend Snippet: Multivariate analysis of the glycan profiles of basigin by PCA. In the score plot ( left panel ), the lectin microarray data of endogenous basigin enriched from the non-tumor (blue) and tumor (red) regions of PDAC tissue sections ( N = 14) are plotted. The numbers of three non-tumor samples obtained from O blood group patients (No. 11–13) are indicated. In the loading plot ( right panel ), the relative signal intensities of 45 lectins on the array are plotted. The lectins extracted from the non-tumor and tumor regions are indicated in blue and red, respectively.

Article Snippet: The adjusted Cy3-labeled protein samples were applied to a lectin microarray slide (LecChip TM Ver.1.0; GlycoTechnica Ltd., Yokohama, Japan) prewashed with the probing buffer and incubated for overnight at 20°C with gently shaking to completely form the lectin–glycoprotein complex.

Techniques: Glycoproteomics, Microarray

Journal: Oncotarget

Article Title: The chimeric antibody chLpMab-7 targeting human podoplanin suppresses pulmonary metastasis via ADCC and CDC rather than via its neutralizing activity

doi:

Figure Lengend Snippet: A. Comparison of epitopes of anti-hPDPN mAbs. PLAG, platelet aggregation-stimulating domain; SA, sialic acid; Gal, galactose; GalNAc, N -acetylgalactosamine. The minimum epitopes of NZ-1 and LpMab-7 are MPGAEDD and RIEDL, respectively. B. The hPDPN-CLEC-2 interaction was blocked by NZ-1 but not by LpMab-7. Inhibition assay was performed using ELISA. Purified hPDPN-Fc was immobilized at 1 μg/ml. After blocking, LpMab-7 or NZ-1 was added at 1 μg/ml. The plates were incubated with biotinylated CLEC-2-Fc (1 μg/ml) followed by 1:1000-diluted peroxidase-conjugated streptavidin. The enzymatic reaction was conducted with a 1-Step Ultra TMB-ELISA. The optical density was measured at 655 nm. C. Antibody-overlay lectin microarray. Purified hPDPN was applied to a lectin array. After incubation, human IgG was applied to each well for blocking. Then, biotinylated LpMab-7 and NZ-1 were applied and Cy3-labeled streptavidin was added. The glass slide was scanned using a GlycoStation Reader 1200.

Article Snippet: Then, 100 μl of purified hPDPN (31.25–2000 ng/ml) was applied to a lectin array (LecChip ver1.0; GlycoTechnica, Hokkaido, Japan), including triplicate spots of 45 lectins in each of seven divided incubation baths on the glass slide.

Techniques: Comparison, Inhibition, Enzyme-linked Immunosorbent Assay, Purification, Blocking Assay, Incubation, Microarray, Labeling